isibhengezo_sekhasi

S. typhoid/S.Para typhi Ag Rapid Test Idivayisi

S. typhoid/S.Para typhi Ag Rapid Test Idivayisi

I-S. typhoid/S.I-Para typhi Ag Rapid Test Device (Indle) iyi-lateral flow immunoassay yokutholwa kanye nokuhlukaniswa ngesikhathi esisodwa kwe-Salmonella typhi ne-Salmonella P. Typhoid endle.


Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Isimiso

I-S. typhoid/S.I-Para typhi Ag Rapid Test Device (Indle) iyi-lateral flow chromatographic immunoassay.Ikhasethi lokuhlola liqukethe: 1) i-burgundy colored conjugate pad equkethe i-S.typhoid/S.I-antibody ye-Para typhi ehlanganiswe negolide le-colloid, 2) umugqa wolwelwesi lwe-nitrocellulose oqukethe amabhendi amabili okuhlola (amabhendi e-S. typhoid/S. Para typhi) kanye nebhendi yokulawula (ibhendi C).Ibhendi ye-S. typhoid imbozwe ngaphambilini nge-monoclonal anti- S. typhoid ukuze kutholwe i-S. typhoid Ag, S. Para typhi band imbozwe ngaphambilini ngezinto ezivuselelayo ukuze kutholwe i-S. Para typhi Ag, kanye ne-C band imbozwe ngaphambilini nge-anti mouse IgG.

Uma ivolumu eyanele yesampula yokuhlola ikhishelwa emthonjeni wesampula wekhasethi, isifanekiso sokuhlola sihamba ngesenzo se-capillary kulo lonke ikhasethi lokuhlola.I-S. typhoid Ag uma ikhona kusampula yesiguli izobophezela kuma-conjugate we-S. typhoid Ab.I-immunocomplex ibe isithathwa kulwelwesi yi-anti-typhoid ehlanganiswe ngaphambili ye-S. typhoid, yakhe ibhande le-S. typhoid elinemibala e-burgundy, ekhombisa umphumela wokuhlola we-S. typhoid.

I-S.Para typhi Ag uma ikhona kusifanekiso sesiguli izobophezela kuma-conjugates e-S. Para typhi Ab.I-immunocomplex ibe isithathwa yi-S. Para typhi Ab ehlanganiswe ngaphambili kulwelwesi, yakha ibhande elinombala o-burgundy S. Para typhi Ab, okubonisa umphumela wokuhlola we-S. Para typhi Ab.Ukungabikho kwanoma yimaphi amabhendi okuhlola kuphakamisa umphumela ongemuhle.Ukuhlola kuqukethe isilawuli sangaphakathi (i-C band) okufanele sibonise ibhande elinombala o-burgundy we-immunocomplex ye-goat anti Mouse IgG/Mouse IgG-gold conjugate kungakhathaliseki ukuthuthuka kombala kunoma yimaphi amabhendi okuhlola.

Uma kungenjalo, umphumela wokuhlola awuvumelekile futhi isifanekiso kufanele sihlolwe kabusha ngenye idivayisi.

Inqubo Yokuhlola

Letha izivivinyo, izibonelo, isigcinalwazi kanye/noma izilawuli ekamelweni lokushisa (15-30°C) ngaphambi kokusetshenziswa.

1. Ukuqoqwa kwesampula nokwelashwa kwangaphambili:
1) Khipha futhi ukhiphe isifakeli se-dilution tube.Qaphela ukuthi ungachithi noma uchithe isixazululo eshubhuni.Qoqa ama-specimens ngokufaka uthi lokufakela isicelo okungenani
Izindawo ezi-3 ezihlukene zendle.
2) Buyisela isifakeli eshubhuni bese usigoqa ikepisi ngokuqinile.Qaphela ukuthi ungaphuli ichopho le-dilution tube.
3) Nyakazisa ishubhu lokuqoqa isifanekiso ngamandla ukuze uhlanganise isifanekiso nesigcinalwazi sokukhipha.Izibonelo ezilungiselelwe eshubhuni lokuqoqwa kwesifanekiso zingagcinwa izinyanga eziyisi-6 ku- -20°C uma zingahloliwe phakathi nehora elingu-1 ngemva kokulungiswa.

2. Ukuhlola
1) Khipha isivivinyo esikhwameni saso esivaliwe, bese usibeka endaweni ehlanzekile, esezingeni.Lebula ukuhlolwa ngesihlonzi sesiguli noma sokulawula.Ukuze uthole umphumela omuhle kakhulu, ukuhlolwa kufanele kwenziwe phakathi nehora elilodwa.
2) Ukusebenzisa ucezu lwephepha lethishu, susa ichopho leshubhu lokuhlanjululwa.Bamba ishubhu ngokuqondile bese ukhipha amaconsi angu-3 esixazululo emthonjeni wesifanekiso (S) wedivayisi yokuhlola.
Gwema ukubamba amabhamuza omoya esibonelweni kahle (S), futhi ungawisi noma yisiphi isisombululo efasiteleni lokubuka.
Njengoba ukuhlola kuqala ukusebenza, uzobona umbala unyakaza kulwelwesi.
3. Linda kuze kuvele amabhendi anombala.Umphumela kufanele ufundwe ngemizuzu eyi-10.Ungahumushi umphumela ngemuva kwemizuzu engama-20.


  • Okwedlule:
  • Olandelayo:

  • Bhala umyalezo wakho lapha futhi usithumelele wona